Detection virulence factors of E.coli isolated from diarrhea patients under 2 years

Diarrhea is one of the most common infectious gastroenteritis diseases in the children under two years old. The present study included 100 diarrhea samples from children patients under two years attending to Al-zahraa teaching hospital for Maternity and Children in Najaf during the period from September2013 to February 2014. From these 100 samples : 6o samples gave positive stool culture (causes by bacteria ) , and 40 samples were negative results(causes by other causative agent such parasitic agent , viral agent ,food tolerance and reaction to medicines). Out of 6o samples :30 isolates of E.coli causes diarrhea and 30other bacteria isolates causes diarrhea.The study dealt with Identification of E.coli from diarrheatic patients by using automated VITEK-2 compact system using Gram Negative-Identification (GN-ID) cards and detect some virulence factors of the E.coli such as : hemolysin , proteases, capsule, bioflim production , adhesion. The results explained that all isolates of E.coli have polysaccharide capsules ,it able to produce hemolysin was 51.6% , also it able to produce proteases enzymes was 35.4% ,E.coli able to bioflim formation was 74.2% and E. coli have able to adhere with epithelial cell was 80.6%