Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1,4-galactosyltransferases
- Huang K.
- Parmeggiani F.
- Ledru H.
- Hollingsworth K.
- Mas Pons J.
- Marchesi A.
- Both P.
- Mattey A. P.
- Pallister E.
- Bulmer G. S.
- Van Munster J. M.
- Turnbull W. B.
- Galan M. C.
- Flitsch S. L.
DOIAbstract
Utilising a fast and sensitive screening method based on imidazolium-tagged probes, we report unprecedented reversible activity of bacterial β1,4-galactosyltransferases to catalyse the transgalactosylation from lactose to N-acetylglucosamine to form N-acetyllactosamine in the presence of UDP. The process is demonstrated by the preparative scale synthesis of pNP-β-LacNAc from lactose using β1,4-galactosyltransferase NmLgtB-B as the only biocatalyst
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