theseparationandenrichmentofglycoproteinsorglycopeptidesbasedonnanoparticles

As one of the most important post-translational modifications, protein glycosylation has a significant effect on the structures and functions of proteins. Many low-abundance endogenous glycoproteins/glycopeptides in the serum or tissue extracts are biomarkers with higher clinical sensitivity and specificity, which could provide valuable information to explore the pathogenesis of many diseases, discover disease biomarkers and develop proteins based new drugs. However, it is still a great challenge for glycoproteomics to selectively separate and enrich the glycoproteins/glycopeptides from the complex biological samples due to their extremely low concentrations. Nanoparticle materials have been attracted much attention in the separation and enrichment of glycoproteins/glycopeptides due to their large surface to volume ratio, numerous affinity sites and unique structures. In this work, the recent progress for the separation and enrichment of glycoproteins/glycopeptides based on the nanoparticles, including gold, SiO2, TiO2, nanodiamond and polymer nanoparticles are reviewed. Finally, the challenges and prospects for the methods of the separation and enrichment of glycoproteins/glycopeptides are briefly proposed