Nuclease colicins bind their target receptor in the outer membrane of sensitive cells in the form of a high affinity complex with their cognate immunity proteins. Upon cell entry the immunity protein is lost from the complex by means that are poorly understood. We have developed a sensitive fluorescence assay that has enabled us to study the molecular requirements for immunity protein release. Nuclease colicins use members of the tol operon for their translocation across the outer membrane. We have demonstrated that the amino-terminal 80 residues of the colicin E9 molecule, which is the region that interacts with TolB, are essential for immunity protein release. Using tol deletion strains we analyzed the cellular components necessary for im...
The translocation of protein toxins into a cell relies on a myriad of protein-protein interactions. ...
TolB and Pal are members of the Tol-Pal system that spans the cell envelope of Gram-negative bacteri...
Protein import across cell membranes is an essential life process. Colicins are proteins produced an...
Nuclease colicins bind their target receptor BtuB in the outer membrane of sensitive Escherichia col...
International audienceColicins are bacterial toxins that parasitize OM (outer membrane) receptors to...
International audienceColicins are bacterial toxins that parasitize OM (outer membrane) receptors to...
The natively disordered N-terminal 83-aa translocation (T) domain of E group nuclease colicins recru...
The natively disordered N-terminal 83-aa translocation (T) domain of E group nuclease colicins recru...
We are investigating how protein bacteriocins import their toxic payload across the Gram-negative ce...
International audienceColicin A is a bacterial toxin which forms channels in the cytoplasmic membran...
The natively disordered N-terminal 83-aa translocation (T) domain of E group nuclease colicins recru...
The translocation of protein toxins into a cell relies on a myriad of protein-protein interactions. ...
AbstractTolB and Pal are members of the Tol–Pal system that spans the cell envelope of Gram-negative...
International audienceABSTRACT The Tol-Pal proteins of the cell envelope of Escherichia coli are req...
TolB and Pal are members of the Tol-Pal system that spans the cell envelope of Gram-negative bacteri...
The translocation of protein toxins into a cell relies on a myriad of protein-protein interactions. ...
TolB and Pal are members of the Tol-Pal system that spans the cell envelope of Gram-negative bacteri...
Protein import across cell membranes is an essential life process. Colicins are proteins produced an...
Nuclease colicins bind their target receptor BtuB in the outer membrane of sensitive Escherichia col...
International audienceColicins are bacterial toxins that parasitize OM (outer membrane) receptors to...
International audienceColicins are bacterial toxins that parasitize OM (outer membrane) receptors to...
The natively disordered N-terminal 83-aa translocation (T) domain of E group nuclease colicins recru...
The natively disordered N-terminal 83-aa translocation (T) domain of E group nuclease colicins recru...
We are investigating how protein bacteriocins import their toxic payload across the Gram-negative ce...
International audienceColicin A is a bacterial toxin which forms channels in the cytoplasmic membran...
The natively disordered N-terminal 83-aa translocation (T) domain of E group nuclease colicins recru...
The translocation of protein toxins into a cell relies on a myriad of protein-protein interactions. ...
AbstractTolB and Pal are members of the Tol–Pal system that spans the cell envelope of Gram-negative...
International audienceABSTRACT The Tol-Pal proteins of the cell envelope of Escherichia coli are req...
TolB and Pal are members of the Tol-Pal system that spans the cell envelope of Gram-negative bacteri...
The translocation of protein toxins into a cell relies on a myriad of protein-protein interactions. ...
TolB and Pal are members of the Tol-Pal system that spans the cell envelope of Gram-negative bacteri...
Protein import across cell membranes is an essential life process. Colicins are proteins produced an...