Quantitative proteomics of parotid saliva from patients with periodontal disease

PLEASE NOTE: This work is protected by copyright. Downloading is restricted to the BU community: please click Download and log in with a valid BU account to access. If you are the author of this work and would like to make it publicly available, please contact open-help@bu.edu.Thesis (MSD) --Boston University, Henry M. Goldman School of Dental Medicine, 2013 (Department of Oral Biology).Includes bibliographic references: leaves 81-88.Change in parotid secretions may correlate with periodontal disease susceptibility and disease state. Comparison of protein compositions of periodontal and healthy parotid saliva may lead us to “predictive" or “diagnostic" biomarkers. In the current study, proteomes of parotid secretions from control heathy individual and periodontal disease patient groups were compared by quantitative MS analysis. Different techniques were used in order to achieve the best results. The present study coupled MS with Isotope Coded Affinity Tag (ICAT) and amine-specific Tag for Relative and Absolute quantitation (mTRAQ) 1abeling methods for the identification and relative quantification of proteins in parotid secretion of patients with moderate to severe periodontal disease and compared the results with samples from the control healthy group. Total 83 proteins were identified. 45 proteins were identified by mTRAQ labeling and 41 proteins were identified by ICAT. But only 3 proteins were identified by both ICAT and mTRAQ.This demonstrate the importance of using two different labeling reagent with different chemistries and specificities which would lead to increasing the identification and quantitation of proteins and hence give us a more comprehensive picture of the samples