In vitro plantlet regeneration from nodal segments of creeping jenny (lysimachia nummularia l.) - a medicinal aquatic plant.

WOS:000354156600012Creeping jenny (Lysimachia nummularia L.) is an aquatic medicinal plant of Myrsinaceae family. The study presents the axillary shoot regeneration from shoot tip and 1st and 2nd nodal segment explants cultured on agar solidified MS (Murashige and Skoog) medium containing 0.25-1.25 mg/L BA (6-benzylaminopurine), with or without 0.25 mg/L NAA (alpha-naphthaleneacetic acid). Shoot regeneration frequency of shoot tip, 1st and 2nd nodal segment explants ranged between 58.33-83.33%, 33.33-83.33% and 41.67-91.67%, respectively, whereas shoots per explant of shoot tip, 1st and 2nd nodal segments were recorded to be 2.75-9.30, 2.9-8.94 and 2.13-8.11, respectively. Maximum number of shoots from all explants were scored on MS medium supplemented with 1.25 mg/L BA. Contrarily, minimum number of shoots per explants were scored on MS medium with 1.25 mg/L BA plus 0.25 mg/L NAA. Shoot length ranged between 1.10-2.12 cm, 1.04-2.25 cm and 0.96-2.33 cm (Table 3) for shoot tip, 1st and 2nd nodal segment, respectively. Shoot length of all explants decreased with increased BA concentration, irrespective of NAA in the culture medium. All explants generated plantlets (shoots with rooting) directly on all regeneration media, irrespective of explant type. Regenerated plantlets were successfully acclimatized in the aquariums without showing any negative sign on the growth of plants.Karamanoglu Mehmetbey University through Scientific Research Project commission (BAP)Karamanoglu Mehmetbey University [50-M-12]The authors acknowledge the financial assistance by the Karamanoglu Mehmetbey University through the Scientific Research Project commission (BAP) for funding project number 50-M-12