Detection of black-foot and Petri disease pathogens in soils of grapevine nurseries and vineyards using bait plants

[EN] Little information is currently available regarding the number of species of black-foot and Petri disease pathogens present in soil and their capacity to infect grapevine roots and reach the xylem vessels. Seedlings of grapevine rootstock 41-B, and cvs. Bobal and Palomino were planted both in pots containing soil samples collected from commercial vineyards and in nursery fields. Roots and xylem vessels were later analyzed for fungal isolation. Black-foot pathogens: Ilyonectria alcacerensis, I. macrodidyma, I. novozelandica and I. torresensis were frequently isolated from roots of seedlings grown in all soils evaluated, whereas Petri disease pathogens: Cadophora luteo-olivacea, Phaeoacremonium aleophilum, Pm. parasiticum and Phaeomoniella chlamydospora were only isolated from xylem vessels of seedlings grown in nursery soils, with a low incidence. Ilyonectria alcacerensis, I. novozelandica and I. torresensis were isolated for the first time from grapevines in Spain, and Pm. parasiticum and Ca. luteo-olivacea were detected for the first time in nursery soils. Our results confirm nursery and vineyard soils as an important inoculum source for black-foot pathogens and demonstrate the presence of several Petri disease pathogens in nursery soils.This research was financially supported by the Project RTA2010-00009-C03-03 (Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria, INIA, Spain) and the European Regional Development Fund (ERDF). We acknowledge J. M. De la Cruz-Gento, V. Garrigues, P. Lopez-Soriano and V. Palacios for technical assistance, and Dr. B. B. Landa for critically reading the manuscript and making valuable suggestions.Agustí Brisach, C.; Gramaje, D.; García Jiménez, J.; Armengol Fortí, J. (2013). Detection of black-foot and Petri disease pathogens in soils of grapevine nurseries and vineyards using bait plants. Plant and Soil. 364(1-2):5-13. https://doi.org/10.1007/s11104-012-1333-15133641-2Adalat K, Whiting C, Rooney S, Gubler WD (2000) Pathogenicity of three species of Phaeoacremonium spp. on grapevine in California. Phytopathol Mediterr 39:92–99Agustí-Brisach C, Gramaje D, León M, García-Jiménez J, Armengol J (2011) Evaluation of vineyard weeds as potential hosts of black-foot and Petri disease pathogens. Plant Dis 95:803–810Alaniz S, Leon M, García-Jiménez J, Abad P, Armengol J (2007) Characterization of Cylindrocarpon species associated with black-foot disease of grapevine in Spain. Plant Dis 91:1187–1193Alaniz S, Armengol J, León M, García-Jiménez J, Abad-Campos P (2009) Analysis of genetic and virulence diversity of Cylindrocarpon liriodendri and C. macrodidymum associated with black foot disease of grapevine. Mycol Res 113:16–23Aroca A, Raposo R (2007) PCR-based strategy to detect and identify species of Phaeoacremonium causing grapevines diseases. Appl Environ Microbiol 73:2911–2918Aroca A, Raposo R (2009) Pathogenicity of Phaeoacremonium species on grapevines. J Phytopathol 157:413–419Aroca A, Luque J, Raposo R (2008) First report of Phaeoacremonium vitícola affecting grapevines in Spain. Plant Pathol 57:386Aroca A, Gramaje D, Armengol J, García-Jiménez J, Raposo R (2010) Evaluation of the grapevine nursery propagation process as a source of Phaeoacremonium spp. and Phaeomoniella chlamydospora and occurrence of trunk disease pathogens in rootstock mother vines in Spain. Eur J Plant Pathol 126:165–174Bertelli E, Mugnai L, Surico G (1998) Presence of Phaeoacremonium chlamydosporum in apparently healthy rooted grapevine cuttings. Phytopathol Mediterr 37:79–82Cabral A, Groenewald JZ, Rego C, Oliveira H, Crous PW (2012a) Cylindrocarpon root rot: multi-gene analysis reveals novel species within the Ilyonectria radicicola species complex. Mycol Prog. doi: 10.1007/s11557-011-0777-7Cabral A, Rego C, Nascimento T, Oliveira H, Groenewald JZ, Crous PW (2012b) Multi-gene analysis and morphology reveal novel Ilyonectria species associated with black foot disease of grapevines. Fungal Biol 116:62–80Chaverri P, Salgado C, Hirooka Y, Rossman AY, Samuels GJ (2011) Delimitation of Neonectria and Cylindrocarpon (Nectriaceae, Hypocreales, Ascomycota) and related genera with Cylindrocarpon-like anamorphs. Stud Mycol 68:57–78Crous PW, Gams W (2000) Phaeomoniella chlamydospora gen. et comb. nov., a causal organism of Petri grapevine decline and esca. Phytopathol Mediterr 39:112–188Crous PW, Groenewald JZ, Risede JM, Hywel-Jones NL (2004) Calonectria species and their Cylindrocladium anamorphs: species with sphaeropedunculate vesicles. Stud Mycol 50:415–429Damm U, Fourie PH (2005) A cost-effective protocol for molecular detection of fungal pathogens in soil. S Afr J Sci 101:135–139Dhingra OD, Sinclair JB (1995) Basic plant pathology methods, 2nd edn. CRC Press, Boca RatonDupont J, Laloui W, Magnin S, Larignon P, Roquebert MF (2000) Phaeoacremonium viticola, a new species associated with Esca disease of grapevine in France. Mycologia 92:499–504Edwards J, Pascoe IG (2004) Occurrence of Phaeomoniella chlamydospora and Phaeoacremonium aleophilum associated with Petri disease and esca in Australian grapevines. Australas Plant Pathol 33:273–279Eskalen A, Rooney SN, Gubler WD (2001) Detection of Phaeomoniella chlamydospora and Phaeoacremonium spp. from soil and host tissue with nested-PCR. Phytopathol Mediterr 40:S480Eskalen A, Feliciano AJ, Gubler WD (2007) Susceptibility of grapevine pruning wounds and symptom development in response to infection by Phaeoacremonium aleophilum and Phaeomoniella chalmydospora. Plant Dis 91:1100–1104Fourie PH, Halleen F (2006) Chemical and biological protection of grapevine propagation material from trunk disease pathogens. Eur J Plant Pathol 116:255–265Gams W (2000) Phialophora and some similar morphologically little-differentiated anamorphs of divergent ascomycetes. Stud Mycol 45:187–199Gardes M, Bruns TD (1993) ITS primers with enhanced specificity for Basidiomycetes: application to the identification of mycorrhizae and rusts. Mol Ecol 2:113–118Gramaje D, Armengol J (2011) Fungal trunk pathogens in the grapevine propagation process: potential inoculum sources, detection, identification, and management strategies. Plant Dis 95:1040–1055Gramaje D, Alaniz S, Pérez-Sierra A, Abad-Campos P, García-Jiménez J, Armengol J (2007) First report of Phaeoacremonium mortoniae causing Petri disease of grapevine in Spain. Plant Dis 91:1206Gramaje D, Alaniz S, Pérez-Sierra A, Abad-Campos P, García-Jiménez J, Armengol J (2008) First report of Phaeoacremonium scolyti causing Petri disease of grapevines in Spain. Plant Dis 92:836Gramaje D, Armengol J, Mohammadi H, Banihashemi Z, Mostert L (2009) Novel Phaeoacremonium species associated with Petri disease and esca of grapevines in Iran and Spain. Mycologia 101:920–929Gramaje D, García-Jiménez J, Armengol J (2010) Field evaluation of grapevine rootstocks inoculated with fungi associated with Petri disease and Esca. Am J Enol Vitic 61:512–520Gramaje D, Mostert L, Armengol J (2011) Characterization of Cadophora luteo-olivacea and C. melinii isolates obtained from grapevines and environmental samples from grapevine nurseries in Spain. Phytopathol Mediterr 50:S112–S126Halleen F, Schroers HJ, Groenewald JZ, Crous PW (2004) Novels species of Cylindrocarpon (Neonectria) and Campylocarpon gen. nov. associated with black-foot disease of grapevines (Vitis spp). Stud Mycol 50:431–455Halleen F, Fourie PH, Crous PW (2006a) A review of black-foot disease of grapevine. Phytopathol Mediterr 45:S55–S67Halleen F, Schroers HJ, Groenewald JZ, Rego C, Oliveira H, Crous PW (2006b) Neonectria liriodendri sp. nov., the main causal agent of black foot disease of grapevine. Stud Mycol 55:227–234Halleen F, Fourie PH, Crous PW (2007) Control of black foot disease in grapevine nurseries. Plant Pathol 56:637–645Harrington TC, McNew L (2003) Phylogenetic analysis places the phialophora-like anamorph genus Cadophora in the Heliotales. Mycotaxon 87:141–151Mostert L, Groenewald CJ, Sumerbell RC, Gams W, Crous PW (2006) Taxonomy and pathology of Togninia (Diaporthales) and its Phaeoacremonium anamorphs. Stud Mycol 54:1–115Mugnai L, Graniti A, Surico G (1999) Esca (Black Measles) and brown Wood-streaking: two old and elusive diseases of grapevines. Plant Dis 83:404–418Navarrete F, Abreo E, Martínez S, Bettucci L, Lupo S (2011) Pathogenicity and molecular detection of Uruguayan isolates of Greeneria uvicola and Cadophora luteo-olivacea associated with grapevine trunk diseases. Phytopathol Mediterr 50:S166–S175Oliveira H, Rego MC, Nascimento T (2004) Decline of young grapevines caused by fungi. Acta Hortic 652:295–304Probst C, Jones EE, Ridgway HJ, Jaspers MV (2012) Cylindrocarpon black foot in nurseries-two factors that can increase infection. Australas Plant Pathol 41:157–163Quaglia M, Covarelli L, Zazzerini A (2009) Epidemiological survey on esca in Umbria, central Italy. Phytopathol Mediterr 48:84–91Retief E, McLeod A, Fourie PH (2006) Potential inoculum sources of Phaeomoniella chlamydospora in South African grapevine nurseries. Eur J Plant Pathol 115:331–339Ridgway HJ, Steyaert JM, Pottinger BM, Carpenter M, Nicol D, Steward A (2005) Development of an isolate-specific marker for tracking Phaeomoniella chlamydospora infection in grapevines. Mycologia 97:1093–1101Rolshausen PE, Úrbez-Torres JR, Rooney-Latham S, Eskalen A, Smith RJ, Gubler WD (2010) Evaluation of pruning wound susceptibility and protection against fungi associated with grapevine trunk diseases. Am J Enol Vitic 61:113–119Rooney SN, Eskalen A, Gubler WD (2001) Recovery of Phaeomoniella chlamydospora and Phaeoacremonium inflatipes from soil and grapevine tissues. Phytopathol Mediterr 40:S351–S356Rooney-Latham S (2005) Etiology, epidemiology and pathogen biology of esca disease of grapevines in California. Ph.D. diss. University of California, Davis. Publ. No. AAT 3191148Schroers HJ, Zerjav M, Munda A, Halleen F, Crous PW (2008) Cylindrocarpon pauciseptatum sp. nov., with notes on Cylindrocarpon species with wide, predominantly 3-septate macroconidia. Mycol Res 112:82–92Serra S, Mannoni MA, Ligios V, Fiori PP (2011) Occurrence of Phaeomoniella chlamydospora on grapevine planting material in Sardinia and its control with combined hot water and cyptoconazole treatments. Phytopathol Mediterr 50:S61–S76Sidoti A, Buonocore E, Serges T, Mugnai L (2000) Decline of young grapevines associated with Phaeoacremonium chlamydosporum in Sicily (Italy). Phytopathol Mediterr 39:87–91Tegli S, Bertelli E, Surico G (2000) Sequence analysis of ITS ribosomal DNA in five Phaeoacremonium species and development of a PCR-based assay for the detection of P. chlamydosporum and P. aleophilum in grapevine tissue. Phytopathol Mediterr 39:134–149Whiteman SA, Jaspers MV, Stewart A, Ridgway HJ (2002) Detection of Phaeomoniella chlamydospora in soil using species-specific PCR. N Z Plant Prot 55:139–14