Ensiled citrus pulp as a by-product feedstuff for finishing pigs: nutritional value and effects on intestinal microflora and carcass quality

[EN] Forty pigs of 76.8 +/- 4.2 kg body weight were fed four different diets varying in ensiled citrus pulp (ECP) inclusion level (0, 50, 100, and 150 g of ECP/kg of diet on dry matter base). The trial lasted 5 weeks. During the last week, faecal samples were obtained to calculate apparent nutrient digestibility; also volatile fatty acids (VFA) content in faeces was determined. The digestible energy and protein of ECP was estimated by regression analysis. During the trial faecal samples were collected to determine enterobacteria and lactobacilli counts. At slaughter, carcass characteristics were registered. The inclusion of ECP in the diets decreased energy digestibility but increased neutral and acid detergent fibre digestibility linearly (p3.0.co;2-cIvarsson, E., Liu, H. Y., Dicksved, J., Roos, S., & Lindberg, J. E. (2012). Impact of chicory inclusion in a cereal-based diet on digestibility, organ size and faecal microbiota in growing pigs. animal, 6(7), 1077-1085. doi:10.1017/s1751731111002709Jensen BB, Jørgensen H, 1994. Effect of dietary fiber on microbial activity and microbial gas production in various regions of the gastrointestinal tract of pigs. Appl Environ Microbiol 60: 1897–1904.Jørgensen, H., Zhao, X.-Q., & Eggum, B. O. (1996). The influence of dietary fibre and environmental temoperature on the development of the gastrointestinal tract, digestibility, degree of fermentation in the hind-gut and energy metabolism in pigs. British Journal of Nutrition, 75(3), 365-378. doi:10.1079/bjn19960140Jouany JP, 1982. Volatile fatty acid and alcohol determination in digestive contents, silage juices, bacterial cultures and anaerobic fermentor contents. Sci Aliment 2: 131-144.Kayouli C, Lee S, 1998. Supplementary feeding for dairy smallholders in Pacific Island Countries: Fiji, Samoa, Vanuatu, Cook Islands, Solomon Islands and Tonga. In: Manual of smallholder milk production in the South Pacific; Lee S, Kennard R, Kayouli C, eds. FAO Sub-Regional Office for the Pacific, Apia (Samoa). pp: 67-101.Kyriazakis, I., & Emmans, G. C. (1995). The voluntary feed intake of pigs given feeds based on wheat bran, dried citrus pulp and grass meal, in relation to measurements of feed bulk. British Journal of Nutrition, 73(2), 191-207. doi:10.1079/bjn19950023Leterme, P., Londoño, A. M., Estrada, F., Souffrant, W. B., & Buldgen, A. (2005). Chemical composition, nutritive value and voluntary intake of tropical tree foliage and cocoyam in pigs. Journal of the Science of Food and Agriculture, 85(10), 1725-1732. doi:10.1002/jsfa.2177Macfarlane, G. T., Gibson, G. R., Beatty, E., & Cummings, J. H. (1992). Estimation of short-chain fatty acid production from protein by human intestinal bacteria based on branched-chain fatty acid measurements. FEMS Microbiology Letters, 101(2), 81-88. doi:10.1111/j.1574-6968.1992.tb05764.xMárquez, M. C., & Ramos, P. (2007). Effect of the inclusion of food waste in pig diets on growth performance, carcass and meat quality. animal, 1(4), 595-599. doi:10.1017/s1751731107685000Pascual, J. M., & Carmona, J. F. (1980). Composition of citrus pulp. Animal Feed Science and Technology, 5(1), 1-10. doi:10.1016/0377-8401(80)90005-xMartínez-Vallespín B, Navarrete C, Martínez-Paredes E, Ródenas L, Cervera C, Blas E, 2011. Determinación de la fibra soluble en detergente neutro: modificaciones del método original. Proc XIV Jornadas sobre Producción Animal; Sanz A et al., eds. May, Monta-ana, Zaragoza. pp: 17-18.Montagne, L., Pluske, J. ., & Hampson, D. . (2003). A review of interactions between dietary fibre and the intestinal mucosa, and their consequences on digestive health in young non-ruminant animals. Animal Feed Science and Technology, 108(1-4), 95-117. doi:10.1016/s0377-8401(03)00163-9NRC, 2012. Nutrient requirements of swine. National Research Council, Nat Acad Press, Washington DC, USA.Noblet, J., & Le Goff, G. (2001). Effect of dietary fibre on the energy value of feeds for pigs. Animal Feed Science and Technology, 90(1-2), 35-52. doi:10.1016/s0377-8401(01)00195-xO’Fallon, J. V., Busboom, J. R., Nelson, M. L., & Gaskins, C. T. (2007). A direct method for fatty acid methyl ester synthesis: Application to wet meat tissues, oils, and feedstuffs. Journal of Animal Science, 85(6), 1511-1521. doi:10.2527/jas.2006-491Oluremi, O. I. A., Okafor, F. N., Adenkola, A. Y., & Orayaga, K. T. (2010). Effect of Fermentation of Sweet Orange (Citrus sinensis) Fruit Peel on its Phytonutrients and the Performance of Broiler Starter. International Journal of Poultry Science, 9(6), 546-549. doi:10.3923/ijps.2010.546.549O'Sullivan TC, Lynch PB, Morrisey PA, O'Grady JF, 2003. Evaluation of citrus pulp in diets for sows and growing pigs. Ir J Agr Food Res 42: 243-253.Rijnen MMJA, Verstegen MWA, Heetkamp MJW, Haaksma J, Schrama JW, 2001. Effect of dietary fermentable carbohydrates on energy metabolism in group-housed sows. J Anim Sci 79: 148-154.Ruiz, U. S., Tomaz, M. C., Pascoal, L. A. F., Watanabe, P. H., Amorim, A. B., Melo, G. M. P., & Daniel, E. (2012). Nutrient digestibility of degermed, dehulled corn, citrus pulp, and soy protein concentrate by barrows. Journal of Animal Science, 90(suppl_4), 170-172. doi:10.2527/jas.53863Stein, H. H., & Shurson, G. C. (2009). BOARD-INVITED REVIEW: The use and application of distillers dried grains with solubles in swine diets. Journal of Animal Science, 87(4), 1292-1303. doi:10.2527/jas.2008-1290Van Kleef, D. J., Deuring, K., & van Leeuwen, P. (1994). Anew method of faeces collection in the pig. Laboratory Animals, 28(1), 78-79. doi:10.1258/002367794781065942Van Soest, P. J., Robertson, J. B., & Lewis, B. A. (1991). Methods for Dietary Fiber, Neutral Detergent Fiber, and Nonstarch Polysaccharides in Relation to Animal Nutrition. Journal of Dairy Science, 74(10), 3583-3597. doi:10.3168/jds.s0022-0302(91)78551-2Van Winsen, R. L., Urlings, B. A. P., Lipman, L. J. A., Snijders, J. M. A., Keuzenkamp, D., Verheijden, J. H. M., & van Knapen, F. (2001). Effect of Fermented Feed on the Microbial Population of the Gastrointestinal Tracts of Pigs. Applied and Environmental Microbiology, 67(7), 3071-3076. doi:10.1128/aem.67.7.3071-3076.2001Villamide, M. J., Garcı́a, J., Cervera, C., Blas, E., Maertens, L., & Perez, J. M. (2003). Comparison among methods of nutritional evaluation of dietary ingredients for rabbits. Animal Feed Science and Technology, 109(1-4), 195-207. doi:10.1016/s0377-8401(03)00177-9Watanabe, P. H., Thomaz, M. C., Ruiz, U. dos S., Santos, V. M. dos, Fraga, A. L., Pascoal, L. A. F., … Faria, H. G. de. (2010). Effect of inclusion of citrus pulp in the diet of finishing swines. Brazilian Archives of Biology and Technology, 53(3), 709-718. doi:10.1590/s1516-89132010000300028Watanabe, P. H., Thomaz, M. C., Ruiz, U. S., Santos, V. M., Masson, G. C. I., Fraga, A. L., … Silva, S. Z. (2010). Carcass characteristics and meat quality of heavy swine fed different citrus pulp levels. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 62(4), 921-929. doi:10.1590/s0102-09352010000400023Williams, B. A., Verstegen, M. W. A., & Tamminga, S. (2001). Fermentation in the large intestine of single-stomached animals and its relationship to animal health. Nutrition Research Reviews, 14(02), 207. doi:10.1079/nrr200127Wood, J. D., & Enser, M. (1997). Factors influencing fatty acids in meat and the role of antioxidants in improving meat quality. British Journal of Nutrition, 78(1), S49-S60. doi:10.1079/bjn19970134Xandé, X., Régnier, C., Archimède, H., Bocage, B., Noblet, J., & Renaudeau, D. (2010). Nutritional values of sugarcane products in local Caribbean growing pigs. animal, 4(5), 745-754. doi:10.1017/s175173110999173xZacharias, B., Kerler, A., & Drochner, W. (2004). The influence of 5% and 10% dietary apple pectin on parameters of fermentation in faeces and caecal digesta of weaning pigs. Archives of Animal Nutrition, 58(2), 149-156. doi:10.1080/0003942041000166750