In situ generation of Co(II) by use of a solid-phase reactor in an FIA assembly for the spectrophotometric determination of penicillamine

[EN] A flow injection analysis (FIA) manifold for the determination of penicillamine in pharmaceutical preparations is proposed. The manifold includes a solid-phase reactor for the in situ production of the derivatizing reagent, Co(II) ion, which forms a coloured complex with penicillamine in an alkaline medium. The reactor is prepared by natural immobilization of cobalt carbonate on a polymer matrix, which endows it with a high mechanical and microbiological stability. The cobalt released by passage of a 5 x 10(-4) Mol l(-1) sulphuric acid stream at a flow-rate of 2.3 ml min(-1) is merged with a volume of 314 mu l of sample containing penicillamine in ammonium-ammonia buffer at pH 9.5 to measure the absorbance at 360 nm. Beer's law is obeyed over the penicillamine concentration range 5-60 mg l(-1). The limit of detection (LOD) of the method is 1 mg l(-1) and its throughput 70 samples h(-1). (c) 2005 Elsevier B.V. All rights reserved.Gómez-Taylor Corominas, B.; Pferzschner, J.; Catalá Icardo, M.; Lahuerta Zamora, L.; Martínez Calatayud, J. (2005). In situ generation of Co(II) by use of a solid-phase reactor in an FIA assembly for the spectrophotometric determination of penicillamine. Journal of Pharmaceutical and Biomedical Analysis. 39(1-2):281-284. doi:10.1016/j.jpba.2005.02.044281284391-