The effects of beta-carotene and vitamin E on erythrocytes lipid peroxidation in beta-thalassemia patients

<strong>BACKGROUND</strong>: Thalassemia is the most common hereditary disease in the world. Thalassemic erythrocytes are exposed to higher oxidative stress and lipid peroxidation. The aim of this study was to investigate the effects of beta-carotene and vitamin E on erythrocytes lipid peroxidation in beta-thalassemia patients.<br /> <strong>METHODS</strong>: A prospective double-blind, placebo-controlled study of the effect of beta-carotene and vitamin E on lipid peroxidation in erythrocytes membranes was performed on 120 beta-thalassemia major patients in four groups. The patients were supplemented for 4 weeks as follows: group 1 with beta-carotene (13 mg/day), group 2 with vitamin E (550 mg/day), group 3 with beta-carotene plus vitamin E and group 4 with placebo. We prepared all capsules for 4 roups in the same shape and color. Measurements of serum beta-carotene and vitamin E were performed by high performance<br /> liquid chromatography. After preparation of ghost cells from blood specimens, malondialdehyde (MDA) was determined as index of lipid peroxidation in erythrocytes membranes before and after treatment. RESULTS: The levels of serum beta-carotene and vitamin E were significantly lower and MDA concentrations in erythrocytes membranes were significantly higher in beta-thalassemia patients compared to controls (P<0.001). In groups that treated with vitamin supplements for 4-weeks, lipid peroxidation rates were significantly reduced after treatment (P<0.001), but in placebo group there was not significant difference (P>0.05). <br /> <strong>CONCLUSIONS</strong>: Our findings provide evidence that an oral treatment with beta-carotene and vitamin E can significantly reduce lipid peroxidation of erythrocytes membranes and could be useful in management of beta-thalassemia major patients. <strong>KEYWORDS</strong>: Beta-thalassemia major, beta-carotene, vitamin E, malondialdehyde, lipid peroxidation.<br /&gt