Quantification and Purification of Mangiferin from Chinese Mango (<em>Mangifera indica</em> L.) Cultivars and Its Protective Effect on Human Umbilical Vein Endothelial Cells under H<sub>2</sub>O<sub>2</sub>-induced Stress

Mangiferin is a natural xanthonoid with various biological activities. Quantification of mangiferin in fruit peel, pulp, and seed kernel was carried out in 11 Chinese mango (<em>Mangifera indica</em> L.) cultivars. The highest mangiferin content was found in the peel of Lvpimang (LPM) fruit (7.49 mg/g DW). Efficient purification of mangiferin from mango fruit peel was then established for the first time by combination of macroporous HPD100 resin chromatography with optimized high-speed counter-current chromatography (HSCCC). Purified mangiferin was identified by both HPLC and LC-MS, and it showed higher DPPH<sup>•</sup> free-radical scavenging capacities and ferric reducing ability of plasma (FRAP) than by l-ascorbic acid (Vc) or Trolox. In addition, it showed significant protective effects on human umbilical vein endothelial cells (HUVEC) under H<sub>2</sub>O<sub>2</sub>-induced stress. Cells treated with mangiferin resulted in significant enhanced cell survival under of H<sub>2</sub>O<sub>2</sub> stress. Therefore, mangiferin from mango fruit provides a promising perspective for the prevention of oxidative stress-associated diseases