IDENTIFICATION OF THE ENTOMOPATHOGENIC FUNGI SAMPLE DL0069 BY COMBINATION OF MORPHOLOGICAL AND MOLECULAR PHYLOGENETIC ANALYSES

Species of Simplicillium are biological control agents against certain plant diseases caused by insects and nematodes due to their ability to parasite and kill the host. Recently, this anamorphic genus is classified under Cordycipitaceae as a monophyletic group apart from the type genus Cordyceps. In this current research, we reported the combination of morphological data and molecular phylogenies to identify an entomopathogenic fungal sample (DL0069) found in the mountainous regions of Langbian mountain, Lam Dong Province, Vietnam. Through formation of phialides and conidial chains, DL0069 was most likely a member of Simplicillium genus. From molecular phylogenetic analyses of a portion of the nuclear large ribosomal unit (nrLSU), it was confirmed that DL0069 was most closely related with Simplicillium chinense, a recently found Simplicillium species with a high potency as a biocontrol of nematodes parasiting plants