Bioemulsifier produced by Yarrowia lipolytica using residual glycerol as a carbon source

Bioemulsifier is bioactive molecules produced by different microorganisms with reducing power and surface and interfacial tension. Among the microorganisms producing this molecule is yeast, which can produce different bioemulsifiers in different substrates. Undoubtedly, this biomolecule has excellent potential for industrial applications, but high production costs are the biggest problem in production. Aiming at cost reduction the present study using crude residual glycerol for biosurfactant production by Yarrowia lipolytica. Then isolates were grown in residual glycerol compound medium, rotating 200 rpm at 28ºC for 48 hours. Bioemulsifier production was observed by analysis of dry biomass, pH, surface tension and emulsification index. The results indicated that the emulsion produced from biosurfactant using glycerol as a carbon source by Y. lipolytica has the potential for bioemulsifier production. All isolates obtained similar results for all analyzes, indicating that this species has a linear production among the isolates. Biomass reached 10.08 ± 0.62 g.L-1, there was a sharp drop in pH reaching 4.6, surface tension averaged 41.7 mN.m-1 and emulsification index reached 56%. The isolates tested show potential for bioemulsifier production using glycerol as an unconventional carbon source