The influence of cryogenic grinding on glucosinolates in rapeseed meal

Uljana repica je najraširenija uljarica u Europi koja se u posljednje vrijeme dosta istražuje upravo zbog svojih glukozinolata koji su prisutni u sjemenu. Ti glukozinolati posebno su zanimljivi zbog nutritivnih i antikarcinogenih, ali i antinutritivnih i toksičnih svojstava koji utječu na zdravlje čovjeka. Najznačajniji je oksazolidin-2-tion 2-hidroksi-3-butenil glukozinolat ('progoitrin') koji se nakuplja u sjemenu uljane repice, te dovodi do oštećenja jetre i bubrega. Isprobane su različite metode kako bi se uklonili glukozinolati ili se smanjio njihov sadržaj u svrhu minimiziranja štetnog utjecaja na zdravlje životinja. Istraživanja su usmjerena određivanju sastava, količine, raspodjele glukozinolata u biljkama, njihove fiziološke uloge te potencijalnog biološkog djelovanja. Cilj ovog rada je odrediti udio glukozinolata HPLC-metodom, te vidjeti utjecaj kriogenog mljevenja na njihov sastav. Ovom metodom odredio se sadržaj glukozinolata, te se dokazao utjecaj kriogenog mljevenja na smanjenje udjela glukozinolata.Rapeseed is the most widely spread oil-plant in Europe, which has recently been investigated quite precisely because of its glucosinolates present in the seed. These glucocinolates are particularly interesting due to nutritional and anti-carcinogenic, but also antinutritive and toxic properties that affect human health. The most significant is oxazolidine-2-thione 2-hydroxy-3-butenyl glucosinolate ('progoitrin') that accumulates in rapeseed oil and leads to liver and kidney damage. Various methods were tested to remove glucosinolates or reduce their content to minimize the adverse effect on animal health. Investigations are focused on determining the composition, amount, distribution of glucosinolates in plants, their physiological role and potential biological activity. The aim of this study wasto determine the glucosinolate content by HPLC method, and the influence of cryogenic grinding on theirs composition.By this method the content of glucosinolates was determined, and the effect of cryogenic grinding on their reduction was demonstrated