The effect of hyperbaric oxygenation on plasma angiotensin II levels in healthy Sprague- Dawley rats Kratki opis

Cilj istraživanja: Cilj je ovog istraživanja ispitati utjecaj hiperbarične oksigenacije na plazmatsku razinu angiotenzina II kod zdravih Sprague-Dawley štakora. Nacrt studije: Istraživanje je eksperimentalna (pokusna) studija. Materijali i metode: U istraživanje su bili uključeni zdravi laboratorijski Sprague-Dawley štakori muškog spola, podijeljeni u dvije skupine: Kontrolna skupina (N = 8) - zdravi netretirani štakori te HBO skupina (N = 8) - štakori izloženi intermitentnoj hiperbaričnoj oksigenaciji (četiri dana po dva sata, pod tlakom od 2 bara). Nakon anestezije, štakori su dekapitirani te su prikupljeni uzorci krvi i centrifugirani kako bi se dobila plazma. Iz uzoraka plazme spektrofotometrijskom metodom ELISA određena je koncentracija angiotenzina II. Rezultati: Nisu utvrđene statistički značajne razlike u koncentraciji ANG II između kontrolne i HBO skupine. Zaključak: Hiperbarična oksigenacija, u okviru specifičnog korištenog protokola, ne mijenja značajno razinu angiotenzina II u plazmi zdravih muških Sprague-Dawley štakora.Objective: The objective of this study is to determine the effect of hyperbaric oxygenation on plasma angiotensin II levels in healthy Sprague-Dawley rats. Study design: The study is structured as an experimental study. Material and methods: The study included healthy male Sprague-Dawley lab rats, divided into two groups: Control group (N = 8) - healthy untreated rats and HBOT group (N = 8) - rats exposed to intermittent hyperbaric oxygenation (4 days at 2 hours, under pressure of 2 bar). After anesthesia, the rats were decapitated and blood samples were collected and centrifuged to obtain plasma. Plasma samples were determined by the spectrophotometric method ELISA for concentrations of angiotensin II. Results: No statistically significant difference in ANG II concentration were found between the control and HBO groups. Conclusion: Hyperbaric oxygenation, in the context of the specific protocol that has been used, does not significantly alter plasma angiotensin II levels in healthy male Sprague-Dawley rats