Influence of hyperbaric oxygenation on changes in serum concentrations of low density oxidized lipoproteins and advanced oxidized protein products in Sprague-Dawley rats

CILJ ISTRAŽIVANJA: Cilj ovog istraživanja bio je ispitati promjenu serumske koncentracije naprednih oksidiranih proteinskih proizvoda (AOPP) i oksidiranih lipoproteina niske gustoće (oxLDL) u uvjetima akutne i intermitentne hiperbarične oksigenacije kod zdravih Sprague-Dawley štakora. USTROJ ISTRAŽIVANJA: Eksperimentalna studija na laboratorijskim životinjama. MATERIJALI I METODE: Sprague-Dawley štakori, starosti 9 – 11 tjedana, nasumično su podijeljeni u tri eksperimentalne skupine (N = 6 – 7): kontrola (netretirana skupina), akutna skupina (A-HBO2, štakori izloženi jednom tretmanu hiperbarične oksigenacije te žrtvovani odmah po izlaganju) i intermitentna skupina (4D-HBO2, štakori izloženi djelovanju hiperbaričnog kisika u barokomori 4 uzastopna dana te žrtvovani 5. dan). Tretman hiperbarične oksigenacije provodio se tijekom 2 sata pri tlaku od 2 bara u Rekompresijskoj komori za eksperimente 110 L, Đuro Đaković, Aparati d.d. Nakon provedenog tretmana hiperbarične oksigenacije životinje su izvagane, uspavane te im je izmjeren krvni tlak. Potom su dekapitirane, nakon čega su prikupljeni uzorci krvi iz kojih su izdvojeni serumi za utvrđivanje koncentracije oxLDL i AOPP ELISA metodom. REZULTATI: Tretmani hiperbarične oksigenacije nisu imali utjecaja na promjenu tjelesne mase i krvnog tlaka životinja. Akutno izlaganje hiperbaričnoj oksigenaciji značajno je povećalo serumsku koncentraciju naprednih oksidiranih proteinskih proizvoda (AOPP) u odnosu na kontrolnu i intermitentnu skupinu. Serumska razina oxLDL-a nije se značajno promijenila među ispitivanim skupinama. ZAKLJUČAK: Akutno izlaganje hiperbaričnom kisiku značajno povećava serumsku koncentraciju AOPP-a u odnosu na preostale ispitivane skupine te tako potvrđuje povećanu razinu oksidativnog stresa koja nastaje jednim izlaganjem hiperbaričnoj terapiji bez značajne promjene u koncentraciji oxLDL-a.OBJECTIVE: The aim of this research was to examine the change in serum concentrations of advanced oxidized protein products (AOPP) and low density oxidized lipoproteins (oxLDL) in the conditions of acute and intermittent hyperbaric oxygenation in healthy Sprague-Dawley rats. STUDY DESIGN: Experimental research on laboratory animals conducted as case control study. MATERIALS AND METHODS: Sprague-Dawley rats, 9 to11 weeks old, were randomly divided into three experimental groups (N = 6 to 7): control (untreated group), acute group (A-HBO2, rats exposed to one hyperbaric oxygenation treatment and sacrificed immediately after the exposure) and intermittent group (4D-HBO2, rats exposed to hyperbaric oxygen in the chamber for 4 consecutive days and sacrificed 5th day). The hyperbaric oxygenation treatment was carried out for 2 hours at a pressure of 2 bar in the Recompression chamber for experiments 110 L, Đuro Đaković, Aparati d.d. After the hyperbaric oxygenation treatment, the animals were weighed, sedated and their blood pressure was measured. After they were decapitated, blood samples were collected and serums were extracted to determine oxLDL and AOPP concentrations by ELISA method. RESULTS: Hyperbaric oxygenation treatment did not affect changes in body weight and blood pressure in animals. Acute exposure to hyperbaric oxygenation significantly increased the serum concentration of advanced oxidized protein products (AOPP) in regards to the control and intermittent groups. Serum levels of oxLDL did not change significantly in the observed groups. CONCLUSION: Acute exposure to hyperbaric oxygen significantly increased AOPP serum concentration in regards to the remaining study groups and thus confirms the increased level of oxidative stress produced by one exposure to hyperbaric therapy without a significant change in the oxLDL concentration