The effect of Allium x cornutum and Allium cepa methanolic extracts on survival of HeLa cancer cells

Biljke i biljni derivati potencijalni su izvori biološki aktivnih spojeva koji su sposobni ometati kancerogene procese. Ovoj skupini pripadaju i biljke roda Allium, bogate polifenolima i organosumpornim spojevima s antioksidativnim i antikancerogenim djelovanjem. Cilj rada bio je ispitati utjecaj metanolnih ekstrakata Allium × cornutum i A. cepa na preživljavanje tumorskih HeLa stanica. Stanice su tretirane ekstraktima (koncentracije 100 µg/ml) nakon čega im je određena vijabilnost, te provjerena morfologija i cjelovitost DNA molekule. Oba ekstrakta pokazala su inhibitorni učinak na rast i proliferaciju HeLa stanica. Uočena je promjena morfologije stanica, te fragmentacija DNA molekule nastale kao posljedica apoptoze. Potrebno je provesti ispitivanja na drugim staničnim linijama i in vivo modelnim organizmima kako bi se potvrdio antikancerogeni potencijal Allium esktrakata.Plants and plant derivatives are potential sources of biologically active compounds that are capable of disrupting carcinogenic processes. This group also includes plants of the genus Allium, rich in polyphenols and organosulfur compounds with antioxidant and anticancer effects. The aim of the study was to explore the influence of methanolic extracts of Allium × cornutum and A. cepa on the survival of tumor HeLa cells. The cells were treated with extracts (concentrations of 100 µg/ml), after which their viability was determined, and the morphology and integrity of the DNA molecule. Both extracts showed an inhibitory effect on the growth and proliferation of HeLa cells. Changes in cell morphology and fragmentation of DNA molecules resulting from apoptosis have been observed. Studies on other cell lines and in vivo model organisms need to be performed to confirm the anticancer potential of Allium extracts