Influence of adolescent social instability stress on the intake of ethanol and sucrose in a rodent model

Adolescence is a sensitive period in which the effects of stress and alcohol can have long-lasting impacts. Social instability stress in adolescent rats (SS; postnatal day 30-45, daily 1 hour isolation + new cage partner) alters behavioural responses to psychostimulants and increases anxiety-like behaviour, but differences in voluntary consumption of natural and drug rewards are unknown. The main goal of my thesis was to investigate the effects of adolescent social instability stress (SS) on immediate and long-lasting changes on reward-related behaviours in male rats using voluntary alcohol intake paradigms. Another goal was to investigate the influence of social context on the propensity to drink alcohol, as well as the influence of these factors on sucrose intake. In chapter 2, I found that adolescent SS increased alcohol intake irrespective of social context, and adolescents drank more alcohol than adults. The intake of sucrose was not altered by stress, except during context of competition. In chapter 3, I found that history of alcohol drinking reduced synaptic plasticity markers in the dorsal hippocampus and prefrontal cortex, and this reduction was sometimes further reduced by SS. The propensity to drink alcohol was found not to differ between SS and CTL rats in the first experiment, and reduced among SS rats in the second experiment. After nine days of alcohol absence, the propensity to drink alcohol was not increased by previous alcohol access, and SS increased intake only in alcohol-naïve rats. History of alcohol drinking reduced anxiety-like behaviours and blunted SS-induced reduction in social interactions. Both SS and alcohol decreased corticosterone levels at baseline and after fear recall without changing freezing behaviour. My findings indicate that using a model of mild social stressor can have great impact on adolescent rats, but moderate effects in adult rats. The behavioural changes caused by stress can be enhanced later in life by history of alcohol drinking, but that does not necessarily cause an increase in the propensity to drink during adulthood, as other studies have shown. Adolescent stressed rats drink more alcohol than other groups, but they don’t seem to continue drinking more when they reach adulthood. These results indicate that the effects of social instability stress are transient in regards to propensity to drink, and can be the basis for alterations caused by both alcohol and stress