Molecular Anthropology and the Punta Lobos Assemblage: DNA-Based Sex-Typing of Juveniles From Ancient Hair Samples

The recent proliferation of techniques suitable for DNA recovery in ancient samples has prompted anthropological researchers to explore molecular-based investigations of human remains from archaeological contexts. This study demonstrates the utility of ancient DNA analysis to strengthen a site-specific demographic profile from the Punta Lobos assemblage. DNA extraction via a silica-based extraction technique from juvenile hair bulb samples and further DNA amplification via high cycle-number PCR was undertaken to genetically type individual sex. Morphologically indeterminate subadult samples were typed as biological males when amplification was successful. The inclusion of preliminary amplification results from a mitochondrial DNA marker suggests that DNA concentration in the Punta Lobos hair samples is sufficient for an extended tri-system genetic analysis