Isolation and Quantification of Human Urinary Exosomes by Hydrophobic Interaction Chromatography on PET C-CP Fiber Phase

Exosomes are membranous vesicles ranging from 30-150 nm in diameter, which are secreted by virtually all cell types and found in most bodily fluids, including blood, urine, and saliva, under both healthy and morbid conditions. Exosomes carry diverse cellular constituents from their parent cells including proteins, messenger RNAsmicroRNAs, and DNA. As such, they are important in intracellular communication, and play key roles in many intercellular functions and cancer progression. Furthermore, mounting evidence suggests that tumor cells secrete more exosomes than normal cells. These features have prompted extensive research to isolate and quantify exosomes for prognosis as well as to serve as biomarkers for disease and cancers