A new spectrophotometric method for the determination of cardiovascular drugs in dosage forms

A simple, accurate, and precise spectrophotometric method has been proposed for the determination of three cardiovascular drugs, namely: Atenolol (ATE), Doxazosin mesylate (DOX) and Lisinopril dihydrate (LID) in pharmaceutical formulations. Proposed method is based on the derivatization of drugs with 1,2-naphthoquinone-4-sulfonic (NQS). The optimum experimental conditions have been studied. Beer’s law is obeyed over the concentration of 0.5–3, 0.4–8, and 5–50 μg/mL for ATE, DOX, and LID, respectively. The detection limits were 0.11, 0.12, and 1.16 μg/mL for ATE, DOX, and LID, respectively, with a linear regression correlation coefficient of 0.9993, 0.9998, and 0.9997 and recovery in range from 98.25–102.57, 97.20–100.57, and 97.83–101.80for ATE, DOX, and LID, respectively. Effects of pH, temperature, reaction time, and NQS concentration on the determination of ATE, DOX, and LID, have been examined. This method is simple and can be applied for the determination of ATE, DOX, and LID in pharmaceutical formulations in quality control laboratories