One Point Method for the Assay of the Alternative Complement Pathway Hemolysis in Rats

One point method to assay the functional activity of alternative complement pathway (AP50) was performed using rats sera. As the titration of CH50 or AP50 to see the activity of complement system with ordinary method needs at least 0.1 ml of serum, experiments with small animals find difficulties to assay CH50 and AP50 at the same time. The method in this report is a modification of one point method for CH50, and instrumental in the case of experiments with small animals