First descriptions of fungal pathogens of onions in Puerto Rico were reported by J. A. B. Nolla, J. Matz, R. A.Toro and C.M.Tucker during the 1920s. At that time onions were grown in the northern region of the island and fungal pathogens such as Alternaria allii, Aspergillus niger, Colletotrichum dematium, C. gloeosporioides, Rhizoctonia sp. and Stemphylium botryosum were reported causing important fungal diseases under field conditions. From 1999 to 2000 a broad survey of fungi associated with onion was conducted in the southern region of the island. Pathogenicity tests were conducted with 25 fungal isolates obtained from soil, mature onion bulbs and foliage from fields at Guánica, Juana Díaz, and Santa Isabel, Puerto Rico. The pathogenici...