<ORGINAL ARTICLE>Characterization of human osteosarcoma cell lines : Comparison of phenotypes of cells established from primary and metastatic lung lesions

P(論文)Osteosarcoma is a highly invasive malignant neoplasm which may metastasize to distant sites, particularly the lung. Two osteosarcoma cell lines were established from osteosarcoma tissue obtained from the proximal end of a right tibial tumor, "KIKU" cells and from the lung metastatic lesion of a 27-year-old female patient, "KIKU-M" cells. Investigations of characteristics of human osteosarcoma cell lines from primary and secondary lesions of the same patient have not previously been reported, and to characterize the KIKU and KIKU-M cells, we performed chromosome analysis, examined growth properties, calcification, alkaline phosphatase (ALP) activity, the amount of osteocalcin (BGP), and the invasive capacity. Several abnormal chromosomes not normally present in human metaphases were identified. The doubling time of KIKU-M cells was shorter than that of KIKU cells. The injection of KIKU and KIKU-M cells produced subcutaneous tumors at injection sites after the transplantion. With von Kossa staining, both cell lines showed a definite calcifcaiton in the medium without the addition of ascorbic acid and beta-glycerophosphate. Prominent osteoid formation and califcation were observed in the transplanted osteosarcoma tissue produced by the subcutaneous transplantion of both cell lines. ALP activity in KIKU cells was significantly higher than that in KIKU-M cells and aslo the production of BGP increased more in KIKU cells than in KIKU-M cells. This evidence may show that KIKU-M cells have more immature phenotypic features than KIKU cells in vitro and in vivo. Also, both cell lines were useful in studying human bone metabolism and the mechanism of differentiation and metastasis of human osteosarcoma.departmental bulletin pape