Experimental study of augmentation using glass ceramic granules to apply fibrin adhesive sealing (Tisseel^<[○!R]>)

P(論文)Subperiosteal alveolar ridge augmentation of the mandible of adult dogs was made one month after extraction of the lower P_2, P_3, and P_4. Starting 5 days after the operation, histological observations were continued for 300 days, focusing on the effect of fibrin adhesive sealing, changes in the mandible and augmented mandible, and new bone formation. The newly formed alveolar ridge remained oval for 5 days after the operation. The mandibular ridge was in a stable condition with no movement of the glass ceramic glanules. To confirm the existence of fibrin adhesive, phosphotungstic acid hematoxylin (PTAH) staining was performed. The stain was recognized for 10 days after the operation. After 20 days, however, the stain had disappeared. This led to the conclusion that the fibrin adhesive had been absorbed and disappeared. New bone formation was observed with toluidine blue (TB) staining. New bone and osteoblasts were established on the lingual cortical bone 10 days after the operation. After 20 days, there was bone on the periosteal side too. The bone continued spreading until 60 days after the operation. After 90 days, partial resorption of bone was observed in the buccal cortical bone. After 120 days, the bone had spread into the alveolar ridge. After 150 days, the lingual and buccal sides were connected by new bone. After 300 days, new bone formation had restored the mandibular cortical bone. It is concluded that fibrin adhesive was of great use in stabilizing the glass ceramic glanules in the early stage, with new bone formation in the cortical bone.departmental bulletin pape