In vitro anticoccidial activity of Trachyspermum ammi (Ajwain) extract on oocysts of Eimeria species of Chicken

Background: Due to development of synthetic anticoccidial drug resistance there is improvement of anticoccidial medicines due to which exploration of diverse therapeutic agents is attractive now a days. Amongst original agents’ miscellaneous botanicals have shown encouraging properties against coccidiosis. Present study is also a part of probing innovative therapeutic drugs against coccidiosis which can provide replacement solution to treat coccidiosis.Methods: In current experiment in vitro anticoccidial effect of Trachyspermum ammi (seeds) extract was evaluated. For this purpose, an in vitro sporulation inhibition assay was used. Collected oocysts of four Eimeria species were exposed to six different concentrations (w/v) of T. ammi in 10% Dimethyl sulphoxide solution (DMSO), while Dimethyl sulphoxide and Potassium dichromate solution (K2Cr2O7) served as control groups.Results: Results of study revealed that T. ammi extract showed in vitro anticoccidial effect by affecting on sporulation (%) and damaging (%) Eimeria oocysts in dose dependent manner. T. ammi extract also damaged the morphology of oocysts in terms of shape, size and number of sporocysts.Conclusion: The results strongly support the botanicals applications of T. ammi extract and also demonstrate its potential for use in Poultry coccidiosis control strategies.Keywords: Trachyspermum ammi extract; In vitro; Eimeria; Oocyst