マウスListeria monocytogenes感染に対する酵母マンナンの防御機構について

In order to clarify the mechanisms of the protective effects against Listeria monocytogenes infection in mice of both mannans, a neutral mannan (WNM) and an acidic mannan (WAM025) fractions from bakers\u27 yeast, Saccharomyces cerevisiae, we investigated in vitro bacterial-growth inhibitory activity. The activity of peritoneal phagocytes, macrophages and polymorphonuclear leucocytes, in mice administered with the mannans was enhanced compared with the activity of those from the untreated mice. The activity of the mannan-induced peritoneal phagocytes was inhibited by addition of serine protease inhibitors, diisopropyl fluorophosphate and phenylmethylsulfonyl fluoride. The macrophage activating factor-releasing activity from the mannan-activated spleen lymphocytes was also enhanced compared with that from non-activated spleen lymphocytes