Karakterisasi Simplisia dan Ekstrak Daun Pacar Kuku (Lawsonia inermis Linn) serta Penentuan Kadar Fenolat Total dan Uji Aktivitas Antioksidan

Lawsonia inermis Linn is the plants into the Lythraceae family. This plant has proven to be able to treat a variety of diseases. This research were aimed to identify the characterization of ethanol extracts and simplicia of henna leaves, determine the total phenolic content with Folin-Ciocalteau method and antioxidant activity with Ferric Reducing Antioxidant Power (FRAP). The extraction was performed using 70 % ethanol. Characterization of simplicia was a powder, greenish brown color, bitter tasted and a distinctive odor. The loss of drying simplisia was 4.92 % ± 0.16, total ash was 4.61 % ± 0.02, acid insoluble ash was 4.43 % ± 0.14, water soluble concentration was 3.30 % ± 0.06, and ethanol soluble concentration was 25.46 % ± 0.18. Characterization of ethanol extracts was viscous, brown in color, typical odor and bitter tasted. The water content of ethanol extracts was 9.98 % ± 2.00, total ash content was 2.63 % ± 0.91, and non acid soluble ash content was 0.36 % ± 0.11. The results of the determination of the total phenolics of ethanol extracts was 16.02 g/100 g. And antioxidant activity of ethanol extract was 2.85 mmol Fe (II)/100 gLawsonia inermis Linn merupakan tumbuhan yang termasuk ke dalam famili Lythraceae. Secara farmakologi telah terbukti dapat mengobati berbagai penyakit. Penelitian ini bertujuan untuk mengetahui karakterisasi simplisia dan ekstrak etanol daun pacar kuku, menentukan kadar fenolat total dengan metoda Folin-Ciocalteau dan uji aktivitas antioksidan dengan metode Ferric Reducing Antioxidant Power. Daun pacar kuku diambil di daerah Padang, Sumatera Barat dan dimaserasi menggunakan pelarut etanol 70% hingga didapatkan ekstrak etanol daun pacar kuku. Dari karakterisasi simplisia daun pacar kuku diketahui bahwa bentuknya serbuk, warna coklat kehijauan, rasa pahit dan bau yang khas. Parameter susut pengeringan simplisia diperoleh sebesar 4,92% ± 0,16, kadar abu total 4,61% ± 0,02, kadar abu tidak larut asam 4,43% ± 0,14, kadar sari larut air 3,30% ± 0,06, dan kadar sari larut etanol 25,46% ± 0,18. Karakterisasi ekstrak etanol daun pacar kuku secara organolpetis diketahui bahwa bentuknya cairan kental, warna coklat, rasa kelat dan bau yang khas. Parameter kadar air ekstrak etanol daun pacar kuku didapatkan sebesar 9,98% ± 2,00, kadar abu total 2,63% ± 0,91, dan kadar abu tidak larut asam 0,36% ± 0,11. Hasil penentuan kadar fenolat total ekstrak etanol daun pacar kuku adalah 16,02 g/100g. Aktivitas antioksidan ekstrak daun pacar kuku yaitu 2,85 mmol Fe(II)/100