Add to ORKGThis report describes the construction and characterization of mus-51RIP70, an allele for high-efficiency targeted integration of transgenes into the genome of the model eukaryote Neurospora crassa. Two of the mus-51RIP70 strains investigated in this work (RZS27.10 and RZS27.18) can be obtained from the Fungal Genetics Stock Center. The two deposited strains are, to our knowledge, genetically identical and neither one is preferred over the other for use in Neurospora research
When a gene or an unidentified fragment of DNA from an organism has been cloned, it is often useful...
A mutant gene is most simply transferred from killer to nonkiller by crossing a marked killer strain...
The highly inbred Neurospora crassa strains 74-OR23-1VA (FGSC 2489) and 74-ORS-6a (FGSC 4200) are re...
We report the complementation of the Neurospora crassa pyr-4 mutation with the Aspergillus nidulans ...
Two years ago, Dr. Käfer and Dr. Schroeder independently summarized genetic information on mutagen-s...
We report two new plasmids, pBM60 and pBM61, and procedures to efficiently generate single- copy tra...
The pOKE103 plasmid can be used to transform Neurospora crassa using the pan-2 gene (NCU10048.3) as ...
It is difficult to obtain progeny when N. crassa and N. tetrasperma are intercrossed directly. Metze...
We report the construction of pRAUW122, a plasmid capable of directing the integration of gene-const...
Although nearly sixty temperature-sensitive lesions have been mapped in Neurospora crassa, most of t...
Gene cloning in Neurospora crassa is often achieved by mutant complementation. However, the cloning ...
Eighteen PCR-based markers are described for use in mapping mutations in Oak Ridge background strain...
When a gene or other fragment of DNA is cloned. it is often useful to identify the chrom...
In the asexual cycle of Neurospora crassa, the wild-type strains produce only mycelia and conidia wh...
Disruption of the nonhomologous end-joining (NHEJ) pathway has been shown to increase the efficiency...
When a gene or an unidentified fragment of DNA from an organism has been cloned, it is often useful...
A mutant gene is most simply transferred from killer to nonkiller by crossing a marked killer strain...
The highly inbred Neurospora crassa strains 74-OR23-1VA (FGSC 2489) and 74-ORS-6a (FGSC 4200) are re...
We report the complementation of the Neurospora crassa pyr-4 mutation with the Aspergillus nidulans ...
Two years ago, Dr. Käfer and Dr. Schroeder independently summarized genetic information on mutagen-s...
We report two new plasmids, pBM60 and pBM61, and procedures to efficiently generate single- copy tra...
The pOKE103 plasmid can be used to transform Neurospora crassa using the pan-2 gene (NCU10048.3) as ...
It is difficult to obtain progeny when N. crassa and N. tetrasperma are intercrossed directly. Metze...
We report the construction of pRAUW122, a plasmid capable of directing the integration of gene-const...
Although nearly sixty temperature-sensitive lesions have been mapped in Neurospora crassa, most of t...
Gene cloning in Neurospora crassa is often achieved by mutant complementation. However, the cloning ...
Eighteen PCR-based markers are described for use in mapping mutations in Oak Ridge background strain...
When a gene or other fragment of DNA is cloned. it is often useful to identify the chrom...
In the asexual cycle of Neurospora crassa, the wild-type strains produce only mycelia and conidia wh...
Disruption of the nonhomologous end-joining (NHEJ) pathway has been shown to increase the efficiency...
When a gene or an unidentified fragment of DNA from an organism has been cloned, it is often useful...
A mutant gene is most simply transferred from killer to nonkiller by crossing a marked killer strain...
The highly inbred Neurospora crassa strains 74-OR23-1VA (FGSC 2489) and 74-ORS-6a (FGSC 4200) are re...