Add to ORKGGene cloning in Neurospora crassa is often achieved by mutant complementation. However, the cloning strategy sometimes requires the isolation of a specific genomic region (by chromosome walking) before transformation of N. crassa. This is the case, for example, if the gene to be isolated has a non-selectable phenotype. Here we specifically describe the construction of the cosmid vector, pAC3, which is designed for direct transformation of N. crassa, its utilization for the construction of a genomic library, and chromosome walking in the region of un-10 on linkage group VII
Chromosome walking using ordered genomic libraries is demanding of both labor and materials. Such li...
Chromosome walks have been executed for all seven chromosomes of N. crassa, but the amount of covera...
First adopted as a model organism by Shear and Dodge in 1927, the filamentous fungus Neurospora cras...
Gene cloning in Neurospora crassa is often achieved by mutant complementation. However, the cloning ...
The un-4 gene of Neurospora crassa was cloned to determine the limits of a chromosome walk on linkag...
Chromosome walks from the cpc-1 and Bml loci were extended and the cys-2 locus cloned by sib-selecti...
In microorganisms, genes can often be cloned directly by complementation of mutants with a ge...
Eighteen PCR-based markers are described for use in mapping mutations in Oak Ridge background strain...
The pOKE103 plasmid can be used to transform Neurospora crassa using the pan-2 gene (NCU10048.3) as ...
It is difficult to obtain progeny when N. crassa and N. tetrasperma are intercrossed directly. Metze...
We have constructed a list of N. crassa cDNA clones for which partial sequences exist, toward the go...
Although nearly sixty temperature-sensitive lesions have been mapped in Neurospora crassa, most of t...
When a gene or other fragment of DNA is cloned. it is often useful to identify the chrom...
We report the complementation of the Neurospora crassa pyr-4 mutation with the Aspergillus nidulans ...
When a gene or an unidentified fragment of DNA from an organism has been cloned, it is often useful...
Chromosome walking using ordered genomic libraries is demanding of both labor and materials. Such li...
Chromosome walks have been executed for all seven chromosomes of N. crassa, but the amount of covera...
First adopted as a model organism by Shear and Dodge in 1927, the filamentous fungus Neurospora cras...
Gene cloning in Neurospora crassa is often achieved by mutant complementation. However, the cloning ...
The un-4 gene of Neurospora crassa was cloned to determine the limits of a chromosome walk on linkag...
Chromosome walks from the cpc-1 and Bml loci were extended and the cys-2 locus cloned by sib-selecti...
In microorganisms, genes can often be cloned directly by complementation of mutants with a ge...
Eighteen PCR-based markers are described for use in mapping mutations in Oak Ridge background strain...
The pOKE103 plasmid can be used to transform Neurospora crassa using the pan-2 gene (NCU10048.3) as ...
It is difficult to obtain progeny when N. crassa and N. tetrasperma are intercrossed directly. Metze...
We have constructed a list of N. crassa cDNA clones for which partial sequences exist, toward the go...
Although nearly sixty temperature-sensitive lesions have been mapped in Neurospora crassa, most of t...
When a gene or other fragment of DNA is cloned. it is often useful to identify the chrom...
We report the complementation of the Neurospora crassa pyr-4 mutation with the Aspergillus nidulans ...
When a gene or an unidentified fragment of DNA from an organism has been cloned, it is often useful...
Chromosome walking using ordered genomic libraries is demanding of both labor and materials. Such li...
Chromosome walks have been executed for all seven chromosomes of N. crassa, but the amount of covera...
First adopted as a model organism by Shear and Dodge in 1927, the filamentous fungus Neurospora cras...