Add to ORKGThe qa-2+ gene has been widely used as a selectable marker in Neurospora transformations (Akins and Lambowitz 1985. Mol. Cell. Biol. 5:2272-2278). A plasmid (pMSN1) has been constructed to facilitate the cloning of selected genes into a qa-2+ vector. The system takes advantage of the blue/white screening possible when cloned fragments are inserted into the E. coli lacZ (beta-galactosidase) gene, and also facilitates subsequent sequencing of the cloned genes
In the presence of the anti-microtubule agent benomyl, N. crassa synthesizes and secretes a major 60...
We report the construction of pRAUW122, a plasmid capable of directing the integration of gene-const...
We have constructed 2 tn5-containing plasmids, pLH1 and pLH3, specialized towards mutagenesis of gen...
The qa-2+ gene has been widely used as a selectable marker in Neurospora transformations (Akins and ...
The quinic acid inducible qa-2 promoter of Neurospora crassa has been used to express cloned genes b...
The pOKE103 plasmid can be used to transform Neurospora crassa using the pan-2 gene (NCU10048.3) as ...
Several vectors containing dominant antibiotic resistance markers for the transformation of filament...
In the previous Fungal Genetics Newsletter, we described a series of plasmid vectors constructed car...
We report two new plasmids, pBM60 and pBM61, and procedures to efficiently generate single- copy tra...
The use of lacZ as a reporter gene requires appropriate plasmid constructions to produce fusions of ...
PLEASE SEE SUMMARY pg 49NORMAN H. GILES, N. K. ALTON, M. E. CASE, J. A. HAUTALA, J. W. JACOBSON, S. ...
Polyclonal antibodies to and oligonucleotides derived from N-terminal sequences of media proteins of...
Addition of the appropriate restriction enzyme to linearized transforming DNA has been shown to incr...
Several selectable genes have been reported for construction of filamentous fungal transformation ve...
We report the complementation of the Neurospora crassa pyr-4 mutation with the Aspergillus nidulans ...
In the presence of the anti-microtubule agent benomyl, N. crassa synthesizes and secretes a major 60...
We report the construction of pRAUW122, a plasmid capable of directing the integration of gene-const...
We have constructed 2 tn5-containing plasmids, pLH1 and pLH3, specialized towards mutagenesis of gen...
The qa-2+ gene has been widely used as a selectable marker in Neurospora transformations (Akins and ...
The quinic acid inducible qa-2 promoter of Neurospora crassa has been used to express cloned genes b...
The pOKE103 plasmid can be used to transform Neurospora crassa using the pan-2 gene (NCU10048.3) as ...
Several vectors containing dominant antibiotic resistance markers for the transformation of filament...
In the previous Fungal Genetics Newsletter, we described a series of plasmid vectors constructed car...
We report two new plasmids, pBM60 and pBM61, and procedures to efficiently generate single- copy tra...
The use of lacZ as a reporter gene requires appropriate plasmid constructions to produce fusions of ...
PLEASE SEE SUMMARY pg 49NORMAN H. GILES, N. K. ALTON, M. E. CASE, J. A. HAUTALA, J. W. JACOBSON, S. ...
Polyclonal antibodies to and oligonucleotides derived from N-terminal sequences of media proteins of...
Addition of the appropriate restriction enzyme to linearized transforming DNA has been shown to incr...
Several selectable genes have been reported for construction of filamentous fungal transformation ve...
We report the complementation of the Neurospora crassa pyr-4 mutation with the Aspergillus nidulans ...
In the presence of the anti-microtubule agent benomyl, N. crassa synthesizes and secretes a major 60...
We report the construction of pRAUW122, a plasmid capable of directing the integration of gene-const...
We have constructed 2 tn5-containing plasmids, pLH1 and pLH3, specialized towards mutagenesis of gen...