We have constructed 2 tn5-containing plasmids, pLH1 and pLH3, specialized towards mutagenesis of genes from fungi which are auxotrophic for arginine or sensitive to hygromycin (such as the filamentous fungi Aspergillus nidulans and Magnaporthe grisea.) These plasmids are also a useful means of integrating additional marker genes in the plasmid backbone
Discovering the genes underlying fundamental processes that enable cells to live and reproduce is a ...
Low rates of homologous recombination have broadly encumbered genetic studies in the fungal pathogen...
Recently, transformation of Aspergillus species with vector pAN7-1, conferring resistance to...
We report the construction of pRAUW122, a plasmid capable of directing the integration of gene-const...
In the previous Fungal Genetics Newsletter, we described a series of plasmid vectors constructed car...
We report two new plasmids, pBM60 and pBM61, and procedures to efficiently generate single- copy tra...
P. marneffei has been established as an experimentally amenable system to study morphogenesis and pa...
We have constructed a plasmid library containing cDNAs from Aspergillus nidulans fused to the activa...
Detailed evaluation of gene functions in an asexual fungus requires advanced methods of molecular bi...
Resistance to hygromycin B is an important dominant selectable marker in fungal transformation. Our ...
In filamentous fungi such as Fusarium graminearum, disruption of multiple genes of interest in the s...
This reports a convenient way for tagging a complementing sequence (gene or gene fragment) for use i...
A transformation system utilizing Agrobacterium tumefaciens was developed for targeted gene disrupti...
Dominant transformation markers allow maximum flexibility in the choice of transformation recip...
Several selectable genes have been reported for construction of filamentous fungal transformation ve...
Discovering the genes underlying fundamental processes that enable cells to live and reproduce is a ...
Low rates of homologous recombination have broadly encumbered genetic studies in the fungal pathogen...
Recently, transformation of Aspergillus species with vector pAN7-1, conferring resistance to...
We report the construction of pRAUW122, a plasmid capable of directing the integration of gene-const...
In the previous Fungal Genetics Newsletter, we described a series of plasmid vectors constructed car...
We report two new plasmids, pBM60 and pBM61, and procedures to efficiently generate single- copy tra...
P. marneffei has been established as an experimentally amenable system to study morphogenesis and pa...
We have constructed a plasmid library containing cDNAs from Aspergillus nidulans fused to the activa...
Detailed evaluation of gene functions in an asexual fungus requires advanced methods of molecular bi...
Resistance to hygromycin B is an important dominant selectable marker in fungal transformation. Our ...
In filamentous fungi such as Fusarium graminearum, disruption of multiple genes of interest in the s...
This reports a convenient way for tagging a complementing sequence (gene or gene fragment) for use i...
A transformation system utilizing Agrobacterium tumefaciens was developed for targeted gene disrupti...
Dominant transformation markers allow maximum flexibility in the choice of transformation recip...
Several selectable genes have been reported for construction of filamentous fungal transformation ve...
Discovering the genes underlying fundamental processes that enable cells to live and reproduce is a ...
Low rates of homologous recombination have broadly encumbered genetic studies in the fungal pathogen...
Recently, transformation of Aspergillus species with vector pAN7-1, conferring resistance to...