We report the construction of a Destination Vector, called pJHAM007, for the targeted integration of DNA sequences at the histidine-3 (his-3) locus of Neurospora crassa. pJHAM007 has all the necessary features required to perform a simple, rapid and efficient GATEWAY™ recombinational cloning with an Entry Clone to yield a his-3-gene replacement Destination Vector
Citation: Baker, S. E., Schackwitz, W., Lipzen, A., Martin, J., Haridas, S., LaButti, K., . . . McCl...
Background In plant functional genomic studies, gene cloning into binary vectors for plant transform...
We report the complementation of the Neurospora crassa pyr-4 mutation with the Aspergillus nidulans ...
We report the construction of pRAUW122, a plasmid capable of directing the integration of gene-const...
We report two new plasmids, pBM60 and pBM61, and procedures to efficiently generate single- copy tra...
The pOKE103 plasmid can be used to transform Neurospora crassa using the pan-2 gene (NCU10048.3) as ...
The quinic acid inducible qa-2 promoter of Neurospora crassa has been used to express cloned genes b...
In the previous Fungal Genetics Newsletter, we described a series of plasmid vectors constructed car...
Background: Recombinatorial cloning using the Gateway (TM) technology has been the method of choice ...
Cloning is an important technique in functional genomics, particularly genetics and molecular biolog...
This BBF RFC provides a method in which standardized parts can be easily created for Neurospora cras...
Gene cloning in Neurospora crassa is often achieved by mutant complementation. However, the cloning ...
This report describes the construction and characterization of mus-51RIP70, an allele for high-effic...
The use of lacZ as a reporter gene requires appropriate plasmid constructions to produce fusions of ...
It is difficult to obtain progeny when N. crassa and N. tetrasperma are intercrossed directly. Metze...
Citation: Baker, S. E., Schackwitz, W., Lipzen, A., Martin, J., Haridas, S., LaButti, K., . . . McCl...
Background In plant functional genomic studies, gene cloning into binary vectors for plant transform...
We report the complementation of the Neurospora crassa pyr-4 mutation with the Aspergillus nidulans ...
We report the construction of pRAUW122, a plasmid capable of directing the integration of gene-const...
We report two new plasmids, pBM60 and pBM61, and procedures to efficiently generate single- copy tra...
The pOKE103 plasmid can be used to transform Neurospora crassa using the pan-2 gene (NCU10048.3) as ...
The quinic acid inducible qa-2 promoter of Neurospora crassa has been used to express cloned genes b...
In the previous Fungal Genetics Newsletter, we described a series of plasmid vectors constructed car...
Background: Recombinatorial cloning using the Gateway (TM) technology has been the method of choice ...
Cloning is an important technique in functional genomics, particularly genetics and molecular biolog...
This BBF RFC provides a method in which standardized parts can be easily created for Neurospora cras...
Gene cloning in Neurospora crassa is often achieved by mutant complementation. However, the cloning ...
This report describes the construction and characterization of mus-51RIP70, an allele for high-effic...
The use of lacZ as a reporter gene requires appropriate plasmid constructions to produce fusions of ...
It is difficult to obtain progeny when N. crassa and N. tetrasperma are intercrossed directly. Metze...
Citation: Baker, S. E., Schackwitz, W., Lipzen, A., Martin, J., Haridas, S., LaButti, K., . . . McCl...
Background In plant functional genomic studies, gene cloning into binary vectors for plant transform...
We report the complementation of the Neurospora crassa pyr-4 mutation with the Aspergillus nidulans ...