PRODUCTION OF BIOETHANOL FROM SARGASSUM CRASSIFOLIUM USING SELLULASE ENZYMES FROM TRICHODERMA RESEEI AND ASPERGILLUS NIGER

Indonesia has the potential as the world's largest bioethanol producer. The efforts to produce bioethanol using non-crop raw material are recently developed. Sargassum crassifolium is a promising raw material for biofuel production since containing a high concentration of polysaccharides. The purpose of this study to determine Sargassum crassifolium bioethanol production by using enzymes from Trichoderma reesai and Aspergillus niger. Pre-treatment of Sargassum crassifolium at working pressure of 15 Psi, temperature of 121 0 C and sulphuric acid concentration of 0.2 M increased the reducing sugars concentration up to 0,000558 mg/L. The application of cellulase enzymes from Trichoderma reesai and Aspergillus niger (3:1) helped increasing the reducing sugars concentration up to 1031,67 mg/L Fermentation using a naturally yeast, Saccharomyces cereviceae (JCM 3012) (0.15 g/mL), produced bioethanol up to 0,727 % during fermentation that assisted the degradation of Sargassum crassifolium