Nanotopography controls cell cycle changes involved with skeletal stem cell self-renewal and multipotency

We present data showing that individual skeletal stem cell populations respond to nanoscale topography differently, and that seeding protocols can be optimised to enhance self-renewal allowing nanotopography to be used as a tool for the study of multipotency. Biochemical, transcriptomic and metabolomic protocols were used to investigate roles for mitogen activated protein kinases as a self-renewal / differentiation control switch and implicate control of cell cycle as being critical for maintenance of multipotency in prolonged culture. This has clear implications for production of large-numbers of high quality skeletal stem cells in vitro