Phytochemical analysis by NMR-based metabolomics approach in amaryllidaceae plants

International audienceNatural substances represent a strong potential for drug discovery and NMR-based metabolomics are powerful technique [1-2]. Our work focuses on the NMR-based metabolomics study of species belonging to the family Amaryllidaceae. In our laboratory we studied extensively the biosynthesis of the Amaryllidaceae alkaloids [3-4]. Our models are Leucojum aestivum, L. aestivum 'Gravetye Giant', Narcissus tazetta, Galanthus woronowii and G. elwesii from di erent geographical origins. The metabolome profiles were established both from in vivo bulbs and in vitro bulblets of these plants. These plants have been chosen for their ability to synthesize high value-added molecules such as alkaloids, for example galanthamine and lycorine. We performed one-dimensional and two-dimensional NMR analysis of these species. Qualitative and quantitative assessments of primary and secondary metabolites were determined by direct comparisons of recorded spectra