International audienceFaithful transcription initiation is critical for accurate gene expression, yet the mechanisms underlying specific transcription start site (TSS) selection in mammals remain unclear. Here, we show that the histone-fold domain protein NF-Y, a ubiquitously expressed transcription factor, controls the fidelity of transcription initiation at gene promoters in mouse embryonic stem cells. We report that NF-Y maintains the region upstream of TSSs in a nucleosome-depleted state while simultaneously protecting this accessible region against aberrant and/or ectopic transcription initiation. We find that loss of NF-Y binding in mammalian cells disrupts the promoter chromatin landscape, leading to nucleosomal encroachment over the...
Transcription is governed by DNA-binding proteins that recognize specific sequences in regulatory re...
Histones post-translational modifications (PTMs) are crucial for transcriptional control, defining p...
Gene network transitions in embryos and other fate-changing contexts involve combinations of transcr...
International audienceFaithful transcription initiation is critical for accurate gene expression, ye...
Totipotency of embryonic stem cells (ESCs) is controlled at the transcriptional level by a handful o...
Nucleosome depletion at transcription start sites (TSS) has been documented genome-wide in multiple ...
The regulation of gene transcription requires posttranslational modifications of histones that, in c...
The function of a eukaryotic cell crucially depends on accurate gene transcription to ensure the rig...
NF-Y is an ubiquitous trascription factor comprising three subunits, NF-YA, NF-YB, and NF-YC. NF-YB ...
Understanding the transcriptional regulation of pluripotent cells is of fundamental interest and wil...
SummaryWe describe the results of a genome-wide analysis of human cells that suggests that most prot...
International audienceATP-dependent chromatin remodellers allow access to DNA for transcription fact...
The CCAAT box promoter element and NF-Y, the transcription factor (TF) that binds to it, were among ...
Histone tails have a plethora of different post-translational modifications, which are located diffe...
BACKGROUND: Histone tails have a plethora of different post-translational modifications, which are l...
Transcription is governed by DNA-binding proteins that recognize specific sequences in regulatory re...
Histones post-translational modifications (PTMs) are crucial for transcriptional control, defining p...
Gene network transitions in embryos and other fate-changing contexts involve combinations of transcr...
International audienceFaithful transcription initiation is critical for accurate gene expression, ye...
Totipotency of embryonic stem cells (ESCs) is controlled at the transcriptional level by a handful o...
Nucleosome depletion at transcription start sites (TSS) has been documented genome-wide in multiple ...
The regulation of gene transcription requires posttranslational modifications of histones that, in c...
The function of a eukaryotic cell crucially depends on accurate gene transcription to ensure the rig...
NF-Y is an ubiquitous trascription factor comprising three subunits, NF-YA, NF-YB, and NF-YC. NF-YB ...
Understanding the transcriptional regulation of pluripotent cells is of fundamental interest and wil...
SummaryWe describe the results of a genome-wide analysis of human cells that suggests that most prot...
International audienceATP-dependent chromatin remodellers allow access to DNA for transcription fact...
The CCAAT box promoter element and NF-Y, the transcription factor (TF) that binds to it, were among ...
Histone tails have a plethora of different post-translational modifications, which are located diffe...
BACKGROUND: Histone tails have a plethora of different post-translational modifications, which are l...
Transcription is governed by DNA-binding proteins that recognize specific sequences in regulatory re...
Histones post-translational modifications (PTMs) are crucial for transcriptional control, defining p...
Gene network transitions in embryos and other fate-changing contexts involve combinations of transcr...