Comparison of the effects of feeding Indian fish liver oils supplemented with or without cholesterol and bile salts on certain enzymes in liver, heart and serum of rats

The enzymes viz. glucose-6-P-dehydrogenase (EC 1.1.1.49), cholesterol esterase (EC 3.1.1.13), aspartate amino transferase (EC 2.6.1.1) and alanine amino transferase (2.6.1.2) are intimately related to lipid metabolism. Hence their activities are bound to be affected by the type of dietary fat and substances like bile salts and cholesterol which also influence the lipid metabolism. This relationship between dietary lipid constituent and enzymes was studied in albino rats maintained on diets containing three Indian Shark Liver Oils viz. Waghbeer, Khada mushi and Pisori supple-mented with or without cholesterol and bile salts, Enzyme activities were studied in liver, heart and serum. It was noted that higher unsaturation of dietary fat increased the activity of glucose-6-P-dehydrogenase enzyme while activities of transaminases and chole-sterol esterase were lowered. Addition of cholesterol and bile salts to these diets decreased the activity of glucose-6-P-dehydrogenase and hydrolytic activity of cholesterol esterase. There was increase in the activities of transaminases and esterifying activity of choles-terol esterase due to supplementation with cholesterol and bile salts