Evaluation of Matrix Metaloproteinase 2 (MMP2) Activity in Contact Nickel Dermatitis Using Zymoanalysis in Comparison to Normal Individuals

Background and objectivesMatrix Metaloproteinases (MMPs), produced mainly by fibroblasts, play a major role in wound healing processes. This study aimed to investigate the MMP2 activity in dermal fibroblasts found in chronic contact nickel dermatitis lesions. MethodsIn order to study the role of MMP2 in contact nickel dermatitis, fibroblast from patients and healthy individuals were cultured based on ex-plantation of skin. MMP2 activity was measured in fibroblast culture media using zymoanalysis. Zymograms were then analyzed by quantitative densitometry. MTT assay was also used to evaluate and compare proliferation capacity of fibroblasts in both cell cultures.ResultsThe mean MMP2 activity 6-8 days after ex-plantation was significantly higher in patients fibroblasts than in normal individuals( 1709.2 and 134.35.9 in patients and normals, respectively (p<0.05)). The proliferation capacity of patients' fibroblasts was significantly higher than that of normal cases (3859382816 and 2702616527 respectively, p