A study on biochemical differences among five different groups of rice striped stem borer Chilo suppressalis Walker (Lepidoptera: Pyralidae)

Identification of biodiversity in different rice striped stem borer (Chilo supprressalis) populations is very important to adopt suitable integrated pest management procedures. Larvae were collected from five different regions in north of Iran including Gourabzarmikh (Go), Sheikhmahaleh (Sh), Rasht (Ra), Amol (Am) and Babol (Ba). Activity levels of five enzymes including alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase and alpha-amylase were evaluated in 4th instar larvae. In addition, five non-enzymatic compounds such as glucose, cholesterol, total protein, uric acid and urea were also measured. Amount of measured compounds showed significant differences in all groups except for alanine aminotransferase and aspartate aminotransferase. Hierarchical agglomerative clustering under UPGMA model demonstrated that Ba population had the most genetic distance and was separated from other groups. In the second group, Go population had the most genetic distance from others and two groups of Ra and Sh had the least genetic distances