Biofilm Formation among Clinical and Food Isolates of Listeria monocytogenes
- Joana Barbosa
- Sandra Borges
- Ruth Camilo
- Rui Magalhães
- Vânia Ferreira
- Isabel Santos
- Joana Silva
- Gonçalo Almeida
- Paula Teixeira
DOIAbstract
Objective. A total of 725 Listeria monocytogenes isolates, 607 from various foods and 118 from clinical cases of listeriosis, were investigated concerning their ability to form biofilms, at 4°C during 5 days and at 37°C during 24 h. Methods. Biofilm production was carried out on polystyrene tissue culture plates. Five L. monocytogenes isolates were tested for biofilm formation after being exposed to acidic and osmotic stress conditions. Results. Significant differences (P<0.01) between clinical and food isolates were observed. At 37°C for 24 h, most food isolates were classified as weak or moderate biofilm formers whereas all the clinical isolates were biofilm producers, although the majority were weak. At 4°C during 5 days, 65 and 59% iso...
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