The effects of electromagnetic fields on plasma levels of corticosterone, free-T3, free-T4 malonyldialdehyde in white male rabbit with normal diet and hyperchlostrol diet

The main goal of this study was to evaluate the possible effect of whole-body magnetic field(MF) exposure on the variations of corticosterone, Free-T3, Free-T4 and malonyl dialdehyde inplasma in 48 adult white New Zealand male rabbits. Animals were divided into six groups namely,C1 (normal diet, not exposed), C2 (normal diet, sham exposed), T1 (normal diet, exposed toelectromagnetic field), C3 (high-cholesterol diet, not exposed), C4 (high-cholesterol diet, shamexposed) and T2 (high-cholesterol diet, exposed to electromagnetic field). In eight separateexperiments, sham exposed groups (C2 and C4), were exposed to sham stimulated (withoutelectromagnetic stimulation) for 5 days, 2 hour/day and the rabbits of the treatment groups (T1and T2) were treated with triangular form 10 Hz of electromagnetic field for 5 days, 2 hour/day,while the control groups (C1 and C3) had no any exposure. At the end of the exposure, after a 12-hour fasting period, blood samples were taken and level of corticosterone, Free-T3 and Free-T4were measured by Elisa kits and level of malonyldialdehyde was measured by spectrophotometricmethod. The results indicated that the blood serum levels of Free-T3, Free-T4 and Corticosterone inthe T1 and T2 groups were significantly increased compared to those of their own control groups (P< 0.05). Malonyldialdehyde levels in T2 animals showed a significant decrease compared to that ofanimals of C3 and C4 (P < 0.05). We conclude that 10 Hz pulsed electromagnetic field can alter thelevels of Free-T3, Free-T4 and corticosterone in animals with both normal diet and hyperchlosteroldiet and also alter the amount of malonyldialdehyde in animals with hyperchlosterol diet