Large-scale macroinvertebrate assemblage patterns from least-disturbed wadeable stream sites across the 48 contiguous US states

We quantified the patterns in macroinvertebrate assemblages and their associated environmental gradients from 457 least-disturbed wadeable stream sites across the 48 contiguous United States sampled as part of US EPA’s National Wadeable Stream Assessment. The majority of the variation in assemblage composition at the finest taxonomic resolution was related to substrate size, %fastwater habitat, water chemistry, as well as east-west geographic position and elevation. Sites were classified into 5 groups with cluster analysis, and group membership was predicted from environmental data using classification tree analysis (CTA). CTA correctly classified 69.1% of test sites and indicated that groups were distinguished by east-west location, and by factors distinguishing mountain streams from lowland/plains streams. Eastern and western groups that had similar environmental characteristics had very similar coarse scale taxa composition and convergent taxa traits. Ordinations confirmed that composition patterns using coarse level taxa resolution and taxa traits no longer reflected geographic distinctions, but were only related to non-geographic environmental factors. However, composition patterns based on traits, coarse taxa, and macroinvertebrates identified to the finest practical level were all correlated with the same dominant non-geographic environmental gradients