Somatic embryogenesis in Lolium multiflorum suspension culture

The embryogenic cell suspension was obtained from immature embryos of Lolium multiflorum through a callus culture. Somatic embryogenesis was induced by addition of 2,4-D, dicamba and picloram in 0,5 mg/l concentrations in MS liquid nutrient medium. It was shown that somatic embryos arised from single cells. In globular embryoids, the meristematic cells are characterized by the presence of phytoferritin inclusions in the leucoplasts