Radical Scavenging and Anti Proliferative Activity of Woodfordia Fruticosa Kurz on Human Bladder Carcinoma, 5637 Cell Lines

Antiproliferative effect of ethanol extract from a plant used in folk medicine, Woodfordia fruticosa Kurz to human bladder carcinoma, 5637 cell lines. After incubation for 72 h with varying concentration of extract (0,343, 1,029, 3,086, 9.259, 27.78, 83.33 dan 250 mg/ml), anti proliferative effect was determined by the neutral red assay and reported in terms of cell viability. The ethanol extract, had a significant anti proliferation against 5637 cell lines with IC50 values of 10,8 mg/ml. A high concentration of ethanol extract (10.8 mg/ml) rapidly reduced the number of 5637 cells. At lower concentrations of ethanol extract (0.343, 1.029, 3.086 mg/ml) the number of 5637 cells started to decrease markedly. Moreover, ethanol extract woodfordia fruticosa Kurz was also tested on their antioxidant in vitro activity based on test involving reactions with 2,2-dihenyl-1-picryl-hydrazyl (DPP). Woodfordia fruticosa Kurz showed a high potenz antioxidant effect with IC50 values of 18 mg/ml. This is about the same with that of ascorbic acid as a positive control showed IC50 values of 10mg/ml. But woodfordia fruticosa Kurz can not inhibit free radical nitric oxide with IC50 values 0f 505,47 mg/ml. This is very small in compare with ascorbic acid as a positive control IC 50 values of 45.04 mg/ml