SWAB TECHNIC, IS IT POSSIBLE TO MEASURED PROINFLAMATORY CYTOKINE IN RECURRENT APTHOUS STOMATITIS LESION?

ABSTRACT\ud Background : Recurrent Aphtous Stomatitis is a common oral mucosal disease in human. The exact etiologic of this disease remain unclear with the pro inflamatory cytokine such as TNF-?? may play an important role in this disease. TNF-?? in RAS lesion commonly measured via Peripheral blood of mononuclear cell (PBMC) and via lesional biopsy which is uncomfortable for patient. Aim : to compared the cytokine level that measured via PBMC and swab lesion of RAS. Method : Seven subject diagnosed having ulcer RAS after anamnesis, clinical examination, and full blood count and also have no gastrointestinal or other systemic condition, participated in this study. Ulcer located at symmetrical side and occur no more than 3 days without any prior treatment than being swab using cotton sterile bud and placed in MEM and stored at -20o C until the next test. As a comparing sides, we swabbed the opposite direction of the lesion and also count the cytokine via PBMC that taken from vein blood along with full blood count. Using ELISA tes to measured both. Results : The result by the paired-t test showed statistically significant level of TNF-?? with p = 0.006 and IL-10 with p = 0.014 with each interval confidence 95%. For PBMC comparison, we used Pearson Linear Correlation that showed no significant difference for both cytokine level. Conclusion : From this study we can conclude that swab lesion of RAS may use to measured cytokine level. However, it will need deeper analyze about the cytokine resources