Anti Proliferation effect of creams containing turmeric (curcuma domestic I) and temulawak (curcuma xanthorriza) extract on uvb irradiated epidermal cells of mice (mus musculus)

An investigation about the effectiveness of dermatological creams containing turmeric (curcuma domestica L) and temulawak (Curcuma xanthorriza) extracts againts proliferation on epidermal cell of mice (Mus musculus) induced by UV-B light had been conducted. The purpose of study was to determine the most effective and stable of cream formula as anti-proliferation agent. Turmeric and Temulawak were extracted by maceration method using 70% ethanol as solvent then formulated to make creams dosage form. They were prepared with varios emulsifying agents, i.e. Novomer, vioscolam, and the combination of Span 60 and Tween 60. Various concentrations of Turmeric and extracts (0.5, 1 and 1.5% w/w) in cream dosage form oil in water type were evaluated their stability and effectiveness as anti-proliferation. Stability test of cream was carried out using stress condition method which samples were placed in climatic chamber at 5' C and 35'C by turn for 10 cycles. Furtherore, anti-proliferation effectiveness tes was performed by determining epidermal cell thickness level of mice after treated by creams. UV-B irradiation with dose 400 mJ/cm2 for 6 minutes was used as the proliferation inducer Epidermal cell of mice were evaluated under microscope. The resultd revealed that cream containing 1% Novomer as emulsifying agent was the most stable base cream for turmeric and temulawak extract and cream containing 1,5% turmeric extract was the most effective as anti-proliferation agent