Development and evaluation of multiplex Y-STR assays for application in molecular genealogy
- Jacobs, Manon
- Janssen, Lynn
- Vanderheyden, Nancy
- Bekaert, Bram
- Van de Voorde, Wim
- Decorte, Ronny
Publication date
January 2009
DOI Publisher
Elsevier BV Journal
Forensic Science International Genetics Supplement SeriesAbstract
Three multiplex PCRs were developed for the analysis of 14 single-copy and 4 multi-copy Y chromosome Short Tandem Repeat (STR) loci routinely used by several public genealogical databases. These assays were used in addition to PowerPlex® Y for the analysis of 245 DNA samples from a genealogical project. In total 244 different haplotypes composed of 37 to 40 alleles were identified with one haplotype identical between two males with the same surname. The multi-copy loci DYS464 and DYS724 were the most polymorphic with a gene diversity of at least 0.964. The use of DYS454 and DYS455 can be questioned as these loci had the lowest gene diversity (0.039 and 0.269 respectively).status: publishe
Add to ORKG