Inter-bacterial correlations in subgingival biofilms: a large-scale survey

Aim: Although the complexity of the oral ecology and the ecological differences between health and disease are well accepted, a clear view on the dynamics in relation to disease is lacking. In this study the prevalence and abundance of 20 key oral bacteria was assessed in health and disease and more importantly a closer look was given to the inter-bacterial relationships. Materials and Methods: A blinded microbiological database was analyzed in this cross-sectional, retrospective study. The database was constructed based on microbiological analyses of samples from 6308 patients, with gradations of periodontitis (healthy to periodontitis). Data concerning the abundance of 20 oral bacteria and probing pocket depth was provided. Results: P. gingivalis, T. forsythia, T. denticola, E. nodatum, P. micra and P. intermedia showed a clear increase in abundance and prevalence with increasing pocket depth. Correlation matrices illustrated that almost all microorganisms were in one way correlated to other species and most of these correlations were significant. Several beneficial bacteria showed strong correlations with other beneficial bacteria. Combining several beneficial bacteria could therefore lead to a stronger effect. Conclusion: Knowledge on bacterial correlations can pave the way for new treatment options focusing on restoring the shifted balance.status: publishe