Three-dimensional myocardial strain estimation from volumetric ultrasound: experimental validation in an animal model

Although real-time three-dimensional echocardiography has the potential to allow for more accurate assessment of global and regional ventricular dynamics compared to the more traditional two-dimensional ultrasound examinations, it still requires rigorous testing and validation against other accepted techniques should it breakthrough as a standard examination in routine clinical practice. Very few studies have looked at a validation of regional functional indices in an in-vivo context. The aim of the present study therefore was to validate a previously proposed 3D strain estimation-method based on elastic registration of subsequent volumes on a segmental level in an animal model. Volumetric images were acquired with a GE Vivid7 ultrasound system in five open-chest sheep instrumented with ultrasonic microcrystals. Radial (eRR), longitudinal (eLL) and circumferential strain (eCC) were estimated during four stages: at rest, during esmolol and dobutamine infusion, and during acute ischemia. Moderate correlations for eLL (r=0.63; p